FIGURE 2.

Cellular toxicity and cell migration in response of AgNPs. (A) Cytotoxicity of AgNPs on RBL cells were determined by MTT assay. MTT salt gets reduced by reducing enzymes into water-insoluble formazan. The images of the cells with AgNPs showed the dosage dependent effect of the AgNPs (1–10 μg/mL). (B) Determination of IC₅₀ value (estimated to be 3.40 μg/mL) of AgNPs on RBL cells (data represented as SEM). (C) Scratch was made onto a monolayer of RBL cells and treated by 3 μg/mL of AgNPs (denoted as T, while the control as C) for different time points. Comparison of migration in both C and T was made by taking images at different time intervals (0, 24, 48, and 72 h) [control -(i), (ii), (iii), (iv) and test -(v), (vi), (vii), (viii)]. Results represented by marking the scratch with parallel lines and visually displaying the number of cells migrated in to the scratch area. The scale bar in (A,C) represents 100 μm.