FIGURE 4.

RT-qPCR analysis of transcript levels of GmEIN3A;1, GmEIN3B;1 and GmEIN3C;1; as well as a predicted downstream target of EIN3, GmERF056, with ethylene pathway stimulation (1 mM ACC, 1.38 mM ethephon) or inhibition (1 μM AVG, 100 ppm 1-MCP, 1 mM AgNO₃) after 2 days at either control or cold temperatures. Transcripts normalized to GmUNK1 transcript levels. Position of the consensus EIN3 binding elements present in the promoter of GmERF056 is displayed above the graph. All promoters depicted in Figure 4–6 were examined for the consensus binding elements shown. Error bars represent SD. One way ANOVA was performed for each temperature individually (Supplementary Table S3). Post-hoc analysis using TukeyHSD was performed comparing treatment with the mock from the same temperature, ^∗p < 0.05, ^∗∗p < 0.01. Comparison between cold treated and non-cold treated mocks was done using Student’s t-test, ^∧p < 0.05. n = 3 composed of a total of 18–21 plants.