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. 2019 Feb;10(1):20–24. doi: 10.24171/j.phrp.2019.10.1.05

Table 1.

Random amplified polymorphic DNA-polymerase chain reaction cycle programs.

Temperature Time
94°C 45 s
30°C 120 s
72°C 60 s
4 cycles
94°C 5 s
30°C 30 s
72°C 30 s
25 cycles
72°C 10 min