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. 2019 Feb 25;14:1469–1487. doi: 10.2147/IJN.S191340

Table S3.

Differential expression of proteins involved in the macromolecular synthesis identified by TMT-labeled quantitative proteomics in Pseudomonas aeruginosa

Protein description PA + AgNPsa PAb PA + AgNPs/PA ratio P-value
Acetyl-coenzyme A carboxylase carboxyl transferase 0.853±0.007 1.133±0.028 0.753 4.4416E–5
subunit alpha
Enoyl-[acyl-carrier-protein] reductase (NADH) 0.742±0.028 1.253±0.009 0.592 2.001E–5
3-Oxoacyl-[acyl-carrier-protein] reductase 0.848±0.006 1.160±0.024 0.731 1.73857E–5
3-Oxoacyl-[acyl-carrier-protein] synthase 2 0.846±0.008 1.152±0.006 0.734 4.453E–7
3-Hydroxydecanoyl-[acyl-carrier-protein] dehydratase 0.857±0.031 1.126±0.032 0.761 0.00052071
Malonyl CoA-acyl carrier protein transacylase 0.785±0.012 1.224±0.048 0.641 5.6695E–5
Beta-ketoacyl-[acyl-carrier-protein] synthase 0.850±0.026 1.141±0.025 0.745 0.000175211
Acetyl-CoA acetyltransferase 1.135±0.007 0.871±0.003 1.303 1.3897E–7
Probable acyl-CoA thiolase 1.135±0.015 0.867±0.008 1.309 4.5E–6
Ribonucleoside-diphosphate reductase 0.797±0.013 1.199±0.028 0.665 1.84241E–5
Polyribonucleotide nucleotidyltransferase 0.839±0.015 1.161±0.019 0.722 2.0224E–5
Adenylosuccinate lyase 0.788±0.024 1.205±0.024 0.654 3.7295E–5
Phosphoribosylglycinamide formyltransferase 0.875±0.047 1.137±0.057 0.769 0.0035588
Bifunctional purine biosynthesis protein 0.785±0.010 1.218±0.023 0.644 2.3803E–6
Probable purine/pyrimidine phosphoribosyl transferase 0.752±0.014 1.258±0.003 0.598 0.00044469
Phosphoribosylformylglycinamidine cyclo-ligase 0.845±0.033 1.144±0.041 0.739 0.00055562
Phosphoribosylamine-glycine ligase 0.770±0.003 1.234±0.021 0.624 6.5436E–7
N5-carboxyaminoimidazole ribonucleotide synthase 0.860±0.054 1.145±0.029 0.751 0.00174152
Carbamoyl-phosphate synthase large chain 0.852±0.002 1.146±0.004 0.744 1.1764E–8
Carbamoyl-phosphate synthase small chain 0.823±0.032 1.168±0.017 0.704 0.000137371
Uridylate kinase 0.857±0.007 1.129±0.009 0.759 1.0846E–6
Methylcrotonyl-CoA carboxylase, alpha-subunit (Biotin-containing) 1.140±0.002 0.857±0.015 1.33 0.00129965
Putative 3-methylglutaconyl-CoA hydratase 1.144±0.019 0.868±0.008 1.317 1.77453E–5
7,8-Dihydroneopterin aldolase 0.866±0.036 1.136±0.080 0.762 0.0043613
Folylpolyglutamate synthetase 0.810±0.017 1.193±0.034 0.679 4.2101E–5
30S ribosomal protein S1 0.718±0.009 1.270±0.002 0.565 0.000161656
30S ribosomal protein S2 0.755±0.012 1.250±0.028 0.604 3.1805E–6
30S ribosomal protein S3 0.674±0.006 1.326±0.022 0.508 1.7496E–7
30S ribosomal protein S4 0.697±0.014 1.293±0.002 0.539 0.0003421
30S ribosomal protein S5 0.731±0.013 1.289±0.017 0.567 7.1018E–7
30S ribosomal protein S6 0.744±0.019 1.269±0.025 0.586 4.0811E–6
30S ribosomal protein S7 0.688±0.012 1.304±0.030 0.527 1.4895E–6
30S ribosomal protein S8 0.717±0.010 1.285±0.016 0.558 3.1299E–7
30S ribosomal protein S9 0.694±0.018 1.301±0.027 0.534 2.4156E–6
30S ribosomal protein S10 0.683±0.010 1.320±0.009 0.517 1.1717E–7
30S ribosomal protein S11 0.693±0.010 1.293±0.026 0.536 8.5873E–7
30S ribosomal protein S12 0.675±0.015 1.327±0.021 0.509 8.0841E–7
30S ribosomal protein S13 0.695±0.004 1.310±0.009 0.53 1.1977E–8
30S ribosomal protein S14 0.641±0.005 1.368±0.021 0.469 8.5181E–8
30S ribosomal protein S15 0.71±0.0460 1.281±0.020 0.554 0.000100556
30S ribosomal protein S16 0.765±0.014 1.247±0.018 0.613 1.6871E–6
30S ribosomal protein S17 0.728±0.032 1.237±0.027 0.588 4.2612E–5
30S ribosomal protein S18 0.733±0.024 1.259±0.046 0.582 4.2153E–5
30S ribosomal protein S19 0.673±0.018 1.324±0.015 0.509 1.1598E–6
30S ribosomal protein S20 0.624±0.070 1.361±0.150 0.458 0.00104168
30S ribosomal protein S21 0.654±0.021 1.353±0.038 0.483 3.9375E–6
50S ribosomal protein L1 0.713±0.012 1.286±0.030 0.555 1.8159E–6
50S ribosomal protein L2 0.637±0.005 1.361±0.013 0.468 1.9918E–8
50S ribosomal protein L3 0.706±0.014 1.285±0.014 0.549 6.3875E–7
50S ribosomal protein L4 0.741±0.019 1.257±0.013 0.589 2.4412E–6
50S ribosomal protein L5 0.732±0.015 1.261±0.004 0.58 0.00046125
50S ribosomal protein L6 0.729±0.019 1.266±0.032 0.575 1.59459E–5
50S ribosomal protein L7/L12 0.762±0.021 1.242±0.068 0.614 0.000158888
50S ribosomal protein L9 0.726±0.029 1.278±0.016 0.568 1.987E–5
50S ribosomal protein L10 0.714±0.015 1.269±0.014 0.563 8.4667E–7
50S ribosomal protein L11 0.705±0.014 1.308±0.027 0.539 1.4216E–6
50S ribosomal protein L13 0.761±0.011 1.242±0.019 0.613 1.073E–6
50S ribosomal protein L14 0.741±0.014 1.258±0.011 0.589 7.0989E–7
50S ribosomal protein L15 0.691±0.009 1.314±0.035 0.526 1.388E–6
50S ribosomal protein L16 0.682±0.020 1.311±0.049 0.52 1.90508E–5
50S ribosomal protein L17 0.712±0.077 1.262±0.057 0.564 0.00106437
50S ribosomal protein L18 0.707±0.006 1.311±0.044 0.539 3.4752E–6
50S ribosomal protein L19 0.704±0.012 1.304±0.031 0.54 1.7169E–6
50S ribosomal protein L20 0.709±0.007 1.295±0.031 0.547 1.0428E–6
50S ribosomal protein L21 0.746±0.020 1.257±0.038 0.594 2.1688E–5
50S ribosomal protein L22 0.717±0.008 1.285±0.018 0.558 2.7836E–7
50S ribosomal protein L23 0.707±0.019 1.267±0.044 0.558 2.063E–5
50S ribosomal protein L24 0.718±0.014 1.276±0.033 0.563 3.4807E–6
50S ribosomal protein L25 0.759±0.013 1.238±0.015 0.613 1.0153E–6
50S ribosomal protein L27 0.662±0.015 1.341±0.014 0.494 5.3758E–7
50S ribosomal protein L28 0.730±0.008 1.271±0.039 0.574 3.8545E–6
50S ribosomal protein L29 0.652±0.034 1.329±0.021 0.491 2.06E–5
50S ribosomal protein L30 0.618±0.055 1.385±0.062 0.446 0.000158851
50S ribosomal protein L31 type B 0.843±0.045 1.160±0.055 0.727 0.00152433
50S ribosomal protein L32 0.587±0.032 1.349±0.069 0.435 4.2315E–5
50S ribosomal protein L33 0.659±0.031 1.340±0.074 0.492 7.6482E–5
50S ribosomal protein L34 0.607±0.017 1.391±0.059 0.436 4.8286E–6
50S ribosomal protein L35 0.608±0.011 1.387±0.077 0.438 1.80613E–5
50S ribosomal protein L36 0.689±0.019 1.309±0.020 0.526 1.9408E–6
Ribosome modulation factor 1.743±0.141 0.277±0.004 6.285 1.2295E–6
DNA primase 0.827±0.052 1.179±0.028 0.702 0.00076473
Ribonuclease HII 0.465±0.066 1.529±0.040 0.304 0.000159774
Replicative DNA helicase 0.808±0.023 1.174±0.020 0.688 3.9244E–5
ATP-dependent RNA helicase 0.796±0.011 1.189±0.019 0.669 2.4613E–6
ATP-dependent RNA helicase 0.616±0.008 1.372±0.005 0.449 2.9544E–8
Poly(A) polymerase I 0.854±0.005 1.147±0.045 0.744 0.0058823
Transcription termination factor 0.781±0.009 1.210±0.013 0.645 5.4598E–7
DNA-directed RNA polymerase subunit alpha 0.763±0.004 1.227±0.014 0.622 1.4663E–7
DNA-directed RNA polymerase subunit beta 0.751±0.008 1.246±0.010 0.603 1.3205E–7
DNA-directed RNA polymerase subunit omega 0.699±0.017 1.294±0.010 0.54 9.3888E–7
DNA-directed RNA polymerase subunit beta’ 0.748±0.002 1.249±0.013 0.598 5.5309E–8
Regulatory protein, RsaL 1.160±0.014 0.852±0.031 1.362 0.000141232
Transcriptional regulator, PtxS 1.119±0.024 0.854±0.037 1.311 0.00062403
Two-component response regulator, CopR 1.234±0.050 0.785±0.036 1.572 0.00020184
Two-component response regulator, PprB 1.200±0.095 0.809±0.057 1.483 0.0030848

Notes:

a

P. aeruginosa treated with AgNP;

b

P. aeruginosa without AgNP treatment.

Abbreviations: AgNP, silver nanoparticle; PA, P. aeruginosa; TMT, Tandem Mass Tag.