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. 2019 Feb 19;15(2):e1007915. doi: 10.1371/journal.pgen.1007915

Fig 3. Detection of B. lanceolatum small RNAs.

Fig 3

A. Four libraries were prepared for each biological sample, to detect small RNAs bearing either a single 5′ phosphate (Libraries #1 and 2) or any other number of phosphates (including zero; Libraries #3 and 4), and either a (2′-OH and 3′-OH) or a protected 3′ end (Libraries #1 and 3), or specifically a protected (e.g., 2′-O-methylated) 3′ end (Libraries #2 and 4). hpf: hours post fertilization. B. Size distribution of genome-matching adult male small RNAs, excluding reads that match abundant non-coding RNAs (rRNAs, tRNAs, snRNAs, snoRNAs or scaRNAs). Read numbers are normalized by the total number of genome-matching reads (including <18 nt and >30 nt reads) that do not match abundant non-coding RNAs, and expressed as parts per million (ppm). C. Size distribution of adult male small RNAs matching pre-miRNA hairpins in the sense (blue) or antisense (red) orientation.