Fig 5. Model predictions of viral titers in low MOI conditions.
The mathematical model fitted to measurements obtained from MDCK cell infections at an MOI of 73 (S1 Fig) was used to reproduce results of experiments performed at (A-C) MOI 3 and (D-F) MOI 10−4 based on TCID50. Symbols represent measurements of MDCK cell infections with influenza A/PR/8/34 (H1N1) obtained from NIBSC (A-C) and RKI (D-F). (A+D) Description of the fraction of infectious virions released with different initial conditions. Model prediction for total (B+E) and infectious virus particles (C+F) released. Error bars in (D-F) indicate standard deviations of three individual experiments. Solid lines depict simulations of the extended model with initial virus concentrations adapted to the respective MOI. Dashed lines represent simulations of the extended model with an additional change to the initial conditions to adjust for low MOI scenarios, i.e. by increasing the initial fraction of infectious virions released.