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. Author manuscript; available in PMC: 2020 Jan 17.
Published in final edited form as: J Toxicol Environ Health A. 2019 Jan 17;82(2):99–112. doi: 10.1080/15287394.2019.1566105

Figure 1: Study design.

Figure 1:

At E15, dams were removed from the NIH-07 diet and placed on a purified FeS or a purified FeD diet, which differed only in Fe content (350 ppm vs 35 ppm). Male offspring were exposed via gavage to either 100 mg/kg MnCl2 or isotonic VEH every other day from P4 to P28. MnOE and FeD diet were continued until P28, when offspring were placed back on an NIH-07 diet for the remainder of the experiment. Two or three male pups per litter were included in each experimental group, with a total of 3 litters included.