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. 2019 Mar 1;9:3212. doi: 10.1038/s41598-019-39929-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glycosylation pattern of the Newman, Wood 46 and ATCC55804 WTAs. (A) 500 MHz ¹H NMR spectra of purified WTAs in D₂O at 293 K; (B) HPAEC-PAD chromatograms of GlcNAc-ribitol disaccharides obtained from S. aureus WTA HF-hydrolysis of the Wood 46 strain (β-D-GlcNAc-(1→4)-ribitol), ATCC55804 strain (β-D-GlcNAc-(1→3)-ribitol) and Newman (α-D-GlcNAc-(1→4)-ribitol) strains. GlcNAc: N-acetyl-glucosamine, Ribol: Ribitol, Tris: Tris buffer in samples.