Fig. 2.

Performance of PTex. a RNA interactome capture of HEK293 was used as 100% cross-linked input material and HuR recovery by PTex was assessed. b Quantification of a (HuR n = 6 biologically independent experiments). c Drosophila melanogaster RBP Sxl RBD4 was bound to RNA of several length carrying the Sxl U7 recognition site and UV cross-linked in vitro; minimal RNA length for efficient recovery by PTex is 30 nt. d Quantification of c (n = 5 biologically independent experiments). e PTex recovery of RNA (260 nm) and proteins (280 nm) determined by UV spectroscopy (n = 9; 5 biologically independent experiments; 2 of those with 3 technical repeats each). f, g Relative enrichment of cross-linked HuR (source Fig. 1c) and Sxl (source Fig. 2c, n = 3 biologically independent experiments) by PTex. Error bars represent SD. All data are in Supplementary Data 4. For full blots see Supplementary Figures 10–14