Fig. 5.

Distal appendage assembly on younger mother centrioles. a Average levels of the DAPs and Odf2 associated with younger mother centrioles in RPE-1 during the cell cycle. n = 217 cells for Cep164, 135 for CCDC41, 155 for SCLT1, 176 for FBF1, 244 for Odf2. Box-and-whisker plots of the same dataset are presented in Supplementary Figure 5. Representative results from a single dataset; the quantification was performed several times with similar results (3x for Cep164 and FBF1, 2x for SCLT1, CCDC41 and Odf2). b In prophase, CCDC41 is localized in discrete foci on both the young and old mother centriole. c SCLT1 signal can be detected on maturing centriole from prophase to metaphase, but it is still not organized in nine densities as visible on older mother centrioles. d The levels of Cep164 are low in metaphase and the signal is not structured. Cep164 associated with older mother centriole is less abundant and unstructured than in interphase (compare with Fig. 2b and d). e In telophase, ANKRD26 is associated with the both mother centrioles but is organized in a smaller toroid than in interphase (compare with Fig. 1h). Scale bars: 1 μm for wide-field images; 200 nm for STORM images in (b, c, d) and 500 nm in (e). The source data underlying Figure 5a is provided as a Source Data file