Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 1;9:3312. doi: 10.1038/s41598-019-39842-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Localization of crotamine in kidneys PTECs of mice receiving daily crotamine by ip injections during 21 days. Kidney sections from mice receiving vehicle saline (a–c) or native crotamine (1 µg/animal), daily, for 21 days (d–h). The perinuclear localization of crotamine (red) is shown in the magnified picture (g). The presence of crotamine (red) inside the nuclei was confirmed by the three-dimensional reconstitution of cell nucleus as shown in (h). Differential interference contrast (DIC) with nuclei stained with DAPI (blue) (a and d), and crotamine immunorecognized by rabbit anti-crotamine antibody followed by signal amplification by the Tyramide Signal Amplification (TRITC-labeled tyramide solution, tetramethyl rhodamine system, in red) (b,c,e,f,g and h). Overlay of red and blue fluorescence (c,f,g and h). Bar = 50 μm (a–f) and 5 μm (g, h).