Figure 5.

Cell viability assay for wild-type and Synd-1 deficient HK-2 cells (Synd-1 KD) treated with crotamine. Cytotoxicity of about 50% of wild type HK-2 cells was observed with ~18 µM of crotamine, while for Synd-1 KD cells, concentrations of ~25 µM of crotamine were required. Cytotoxicity for 90% of the wild-type HK-2 cells was observed with ~33 µM of crotamine, while a concentration higher than ~40 µM of crotamine would be required for Synd-1 KD cells. LC₅₀: concentration of drug required to reach 50% of cytotoxicity (dotted line). LC₉₀ concentration of drug required to determine 90% of cytotoxicity (dashed line).