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. 2019 Mar 1;9:3288. doi: 10.1038/s41598-019-39865-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Jurkat T cell on patterned OKT3 antibody surface. (A) OKT3 antibody patterns are used to enrich and immobilize the TCR at specific sites at the plasma membrane of living Jurkat T cells. ZAP70-GFP is recruited to these sites. (B) Typical micropatterned cell showing enrichment of ZAP70-GFP at OKT3/TCR sites. For analysis, selection masks for TCR-enriched (“ON” -red) and -depleted (“OFF” -blue) areas were generated. Scale bar is 5 µm.