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. 2019 Mar 1;9:3288. doi: 10.1038/s41598-019-39865-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FRAP on non-activated Jurkat T cells. (A) A representative FRAP curve of ZAP70-GFP measured in a cell seeded onto a fibronectin-coated surface. A bi-exponential fit (Eq. 2, shown in red) yielded τ₁ = 0.57 s, τ₂ = 17.4 s, f_m = 0.83, b = 0.29 and c = 0.80. A mono-exponential fit (Eq. 1) is shown in blue as a reference. (B) Averaged FRAP curves of ZAP70-GFP measured in cells on a fibronectin-coated surface. Data are from three independent experiments with 17 different cells. The 95% confidence interval is shown. Mean values ± SE from cells fitted individually with Eq. 2 were: τ₁ = 0.29 ± 0.03 s, τ₂ = 12.6 ± 3.9 s, f_m = 0.89 ± 0.02, b = 0.35 ± 0.01 and c = 0.67 ± 0.03. The average of all fit curves is shown in red.