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. 2019 Mar 1;9:3288. doi: 10.1038/s41598-019-39865-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ZAP70-GFP recovery in cells on micropatterned and coated OKT3 surfaces. (A) Recovery of fluorescence over time in “ON” and “OFF” areas in a representative micropatterned cell is shown in red and blue, respectively. Fitting with Eq. 2 yielded τ₁ = 0.2 s, τ₂ = 10.5 s, f_m = 0.90, b = 0.14 and c = 0.7 for the “OFF” curve and τ₁ = 0.3 s, τ₂ = 19.4 s, f_m = 0.77, b = 0.05 and c = 0.32 for the “ON” curve. The relative recovery of fluorescence ΔF over time is shown in black and was fit with Eq. 1 yielding τ = 18.1 s, f_m = 0.73, b = 0.02. (B) Recovery of fluorescence over time in a representative cell on a homogeneously coated OKT3 surface fitted with Eq. 2 yielding τ₁ = 0.26 s, τ₂ = 17.0 s, f_m = 0.77, b = 0.03 and c = 0.47.