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. 2019 Mar 1;9:3288. doi: 10.1038/s41598-019-39865-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of FRAP parameters for cells on micropatterned and homogeneously coated OKT3 surfaces at 37 °C. The fraction of mobile ZAP70-GFP (A,B) and the unbinding time constants τ and τ₂ (C,D) are plotted over the time after seeding for cells on micropatterned (N = 19) (A,C) and homogeneously coated (N = 17) (B,D) OKT3 surfaces. Error bars show the SE of the fit.