Table 1.
Plasmids used to produce the HIV pseudotypes that express the described envelope protein. Pseudotypes were produced by plasmid co-transfections in 293T cells as described by Martínez et al.39.
| Type | Name | Tropism | Subtype | NIH cat# | SHORT ID |
|---|---|---|---|---|---|
| Backbone | pSG3ΔEnv* | 11051 | |||
| Env | pHXB2 | X4 | B | 1069 | HXB2 |
| Env | pAC10.0 | R5 | B | 11024 | AC10 |
| Env | pQ461.e2 | R5 | A | 10460 | Q46 |
| Env | pQ23.17 | R5 | A | 10455 | Q23 |
| Env | pCAP210.2 | R5 | C | 11317 | CAP2 |
| Env | pCM235/GS | R5 | A/E | 7701 | CM235 |
*HIV expression vector that expresses all HIV-1 proteins except Env and Vpu. For all pseudotypes: TZM-bl cells were infected with a virus inoculum corresponding to a RLU/s of 10-fold higher than RLU/s in control cells; RLU/s = Relative Light Units per second.