Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 1;9:3263. doi: 10.1038/s41598-019-40135-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Visualization and image-processing of retinal vasculature. (A) Representative images of retinal flatmounts of Cldn5-eGFP reporter mice stained for CD31 and IB4. Scale bar 200 µm. (B) Representative images of Cldn5-eGFP⁺ retinal flatmounts after transcardial perfusion with tomato Lectin. Scale bar 100 µm. (C) Illustration of the image processing procedure in ImageJ (FIJI). (D) Schematic representation of vascular parameters that are assessed in a retinal flatmount. Vascular area fraction: Ratio of area covered by vasculature to total area in the retina.