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. 2019 Mar 1;9:3263. doi: 10.1038/s41598-019-40135-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Single plexus analysis reveals more accurate measure of vasculature than whole retina analysis. (A) Schematic illustration of whole retina and single plexus analysis. (B) Quantification and comparison of vascular outgrowth, area fraction, length and branching between single plexus and whole retina. (C) Tabular overview of vascular parameters with deviations in %. The animal number is n = 4 (p3, p7, p10) and n = 3 (p30, p60, p120).