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. 2019 Mar 1;9:3270. doi: 10.1038/s41598-019-39400-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phase contrast images of cell morphology through differentiation (EVOS FL Cell Imaging System, scale bar: 1000 µm). (a) Human iPSCs (not at differentiation density), (b) Anterior definitive endoderm at day 6, (c) Hepatic endoderm at day 9, (d) Hepatocyte-like cell maturation at day 16, (e) Hepatocyte-like cell maturation at day 25, and (f) HLCs at day 35.