Fig. 5.

NONO collaborates with SFPQ to suppress recombination at telomeres. a Representative images and explanative models of telomeric sister chromatid exchange (T-SCE) as detected by CO-FISH. b Quantification of T-SCEs in U-2 OS cells transfected with control siRNAs, NONO-specific siRNAs (left panel), or SFPQ-specific siRNAs (right panel). c Quantification of T-SCEs in H1299 cells transfected with control siRNAs, NONO-specific siRNAs (left panel), or SFPQ-specific siRNAs (right panel). d Representative images of CO-FISH experiments performed in U-2 OS cells transfected with indicated siRNAs. e Quantification of T-SCEs in U-2 OS cells transfected with NONO-specific siRNA, or with both NONO- and SFPQ-specific siRNAs compared to control cells. f Quantification of telomere fragility in U-2 OS cells transfected with NONO-specific siRNA, or with both NONO and SFPQ-specific siRNAs. g Representative images of anti-TRF2 and anti-PML co-immunofluorescence to detect APBs in U-2 OS cells transfected with indicated siRNAs. h Quantification of the number of APBs per nucleus of data shown in g. i Representative images of anti-TRF2 and anti-RAD51 co-immunofluorescence in U-2 OS cells transfected with indicated siRNAs. j Quantification of RAD51-TRF2 co-localization events per nucleus of data shown in i. Box plot diagrams (b, c, e, f): middle line represents median; boxes extend from the 25th to 75th percentiles. The whiskers mark the 10th and 90th percentiles. p-values were calculated using a two-tailed Mann–Whitney test. Median T-SCE/fragility values and standard deviation are indicated; n = number of analyzed telomere repeat signals, N = number of analyzed metaphase spreads. Panels h, j means (bars) and standard deviation (error bars) are reported. N = number of independent experiments. n = number of analyzed nuclei. A two-tailed Student’s t-test was used to calculate statistical significance; p-values are shown. Source data is provided as a Supplementary Information File. Scale bar, 1 μm. siRNAs listed in Supplementary Table 1