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. 2019 Mar 1;10:1001. doi: 10.1038/s41467-019-08863-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — NONO and SFPQ regulate telomere length homeostasis in human cancer cells. a Telomere length measurements by quantitative telomere DNA-FISH on interphase U-2 OS cells after transient transfection with indicated siRNAs. Representative images are shown. b Telomere fluorescence intensity was analyzed for each telomere of a. c Telomere length measurements by quantitative telomere DNA-FISH on interphase H1299 cells after transient transfection with indicated siRNAs. Representative images are shown. d Telomere fluorescence intensity analyzed for each telomere of c. e Representative images of combined immunofluorescence using anti-TRF2 and anti-PML antibodies in H1299 cells transfected with SFPQ-specific siRNA or a non-targeting control siRNA. Cells were treated or not treated with 5-aza-2’-deoxycytidine (5-Aza). f Quantification of e; number of TRF2-PML co-localization events per nucleus. Mean values (bars) and standard deviations (error bars) are reported. N = number of independent experiments. n = number of analyzed nuclei. A two-tailed Student’s t-test was used to calculate statistical significance; p-values are shown. Source data is provided as Supplementary Information File. g Representative images of telomere length measurements by quantitative telomere DNA-FISH on interphase H1299 cells transfected with SFPQ-specific siRNA or a non-targeting control siRNA. Cells were treated or not treated with 5-aza-2’-deoxycytidine (5-Aza). h Telomere fluorescence intensity analyzed for each telomere of g. For telomere length measurements, Box plot diagrams (b, d, h): middle line represents median arbitrary fluorescence units (a.f.u.), boxes extend from the 25th to 75th percentiles. The whiskers mark the 10th and 90th percentiles. p-values were calculated using a two-tailed Mann–Whitney test. Median a.f.u. values with standard deviation are indicated; n = number of analyzed telomere repeat signals, N = number of analyzed interphase nuclei. Arbitrary fluorescence units (a.f.u.) are shown. Scale bar, 1 μm. siRNAs listed in Supplementary Table 1