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. 2019 Mar 1;9:3305. doi: 10.1038/s41598-019-39287-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotype of kfo and nco plants is determined by F3′H and CHI genotypes. (a) Representative photos, to scale, and genotyping chromatograms of wild type and yellow-green nco mutants. CHI homozygous recessive mutant genotype results in mutant yellow-green phenotype. Heterozygous and homozygous wild type genotypes result in wild type red phenotype. (b) Representative photos, to scale, and genotyping chromatograms of wild type and pink-green mutant kfoA line plants. F3′H splice site homozygous mutant genotype results in mutant pink-green phenotype. Heterozygous and homozygous wild type genotypes result in wild type red phenotype. Genotypes: +/+, homozygous wild type; +/M, heterozygous mutant; M/M, homozygous mutant. Note single peak in homozygotes, and double peak in heterozygotes at SNP site. Fifteen-week-old plants are shown, grown under cool fluorescent lights in identical conditions.