Table 2.
Genetic diversity and differentiation of foothill and subalpine populations within the target mountain ranges.
| Group | No. of ind./pop. | No. of alleles | No. of private alleles | Allelic richness | HE | HS | DW index | FST | Among populations variation (%) | Pairwise FST among populations (min-max values) | Among groups variation (%) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Jeseníky Mts | |||||||||||
| Subalpine populations | 29/2 | 44 | 0 | 2.61 ± 0.005 | 0.47 ± 0.01 | 0.53 ± 0.015 | 83.61 ± 1.17 | 0.03 | 2.76* | 0.035 | |
| Foothill populations | 31/2 | 53 | 3 | 2.66 ± 0.055 | 0.45 ± 0.01 | 0.44 ± 0.01 | 181.36 ± 59.41 | 0.08 | 7.82*** | 0.057 | |
| Subalpine × foothills | 6.11 (p = 0.33) | ||||||||||
| Krkonoše Mts | |||||||||||
| Subalpine populations | 40/2 | 56 | 5 | 2.57 ± 0.05 | 0.45 ± 0.005 | 0.53 ± 0.005 | 186.06 ± 41 | 0.21 | 21.42*** | 0.132 | |
| Foothill populations | 39/4 | 60 | 3 | 2.69 ± 0.093 | 0.46 ± 0.019 | 0.50 ± 0.035 | 137.28 ± 26.41 | 0.05 | 5.47*** | (0.035) 0.059 (0.08) | |
| Subalpine × foothills | 4.26 (p = 0.14) | ||||||||||
| Tatry Mts | |||||||||||
| Subalpine populations | 31/4 | 68 | 3 | 2.83 ± 0.352 | 0.46 ± 0.038 | 0.58 ± 0.053 | 226.16 ± 44.82 | 0.24 | 24.18*** | (0.065) 0.125 (0.174) | |
| Foothill populations | 32/2 | 87 | 14 | 3.53 ± 0.195 | 0.58 ± 0.04 | 0.6 ± 0.015 | 395.92 ± 15.87 | 0.03 | 3.03 (p = 0.1) | 0.06 | |
| Subalpine × foothills | 2.32 (p = 0.25) | ||||||||||
| Significance of differences between groups of foothill and subalpine populations | F1,12 = 0.09 | F1,12 = 0.09 | F1,12 = 0.3 | F1,12 = 0.08 | |||||||
P-values were estimated by 1000 permutations (*P < 0.05, **P < 0.01, ***P < 0.001).
Additionally, we calculated for subalpine pop. S5 from the Nízké Tatry Mts and foothill pop. F5 and F8 among population variation (subalpine × foothill pop.) = 23.82% (p = 0.33), FST = 0.27, and pairwise FST among populations = (0.06) 0.11 (0.137).