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. 2019 Mar 1;9:3226. doi: 10.1038/s41598-019-39530-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pelo physically interacts with Hbs1. (A–C) Co-expression of Pelo (A, Pelo-RFP, red) and Hbs1 (B, Hbs1-GFP, green) in Drosophila S2 cells, where those two proteins were co-localized in the cytoplasm (C: merged image with DAPI in blue). (D–F) UASp-Pelo-flag (D, red) and UASp-Hbs1-myc (E, green) were both expressed in the cytoplasm of testicular germ cells when they were driven by nosGal4. Note that they could co-localize with each other in the germline cells (F). (G) Pelo interacted with Hbs1 in a yeast two hybrid assay. Scale bar represents 10 μm in A–C and 15 μm in D–F.