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. 2019 Mar 1;9:3289. doi: 10.1038/s41598-019-39772-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative gating strategy for the analysis of VSELs and HSCs. (A) First, an extended lymphocyte gate was created, (B) then cells negative for the Lineage cocktail antigens are gated. (C) Lin- cells were then analyzed for the expression of CD45 and CD34 and (D) CD45 and CD133. (E) Lin-CD34+CD45+ HSCs and Lin-CD34+CD45− VSELs were then plotted on the SSC/FSC dot plot to compare their granularity and size. (F) SSC/FSC dot plot graph showing Lin-CD133+CD45− VSELs and Lin-CD133+CD45+ HSCs.