Table 1.
Sequences of forward and reverse primers of targeted human genes used for qPCR
| Primer Sequences |
|||
|---|---|---|---|
| Gene Name | 5′–3′ Forward | 3′–5′ Reverse | Length |
| Claudin-1 | AGGTGCTATCTGTTCAGTGATG | CTACACAAGGAAAGTCAGCCA | 22,21 |
| Claudin-2 | CCTCCATCCCACTCTTGTTATG | AGAAAGCAGGATGCAGGATG | 22,20 |
| Claudin-3 | CCAAGGCCAAGATCACCAT | GGTTGTAGAAGTCCCGGATAATG | 19,23 |
| Claudin-4 | GCCTTACTCCGCCAAGTATT | AGGGAAGAACAAAGCAGAGAG | 20,21 |
| Claudin-5 | TTGGCTGCTGCCTTACTT | ACCAGCTGTACACATCTTCC | 18,20 |
| Claudin-7 | GCTCTTACCCTAAGTCCAACTC | CTTTCAGGCATCTAGACACTCC | 22,22 |
| Claudin-8 | CTGGGTTCCGAGTTCATTAC | GGCATGGGTTGCCATTAT | 20,18 |
| Claudin-10a | AGACACAGGCTTCTTCCTAGA | GAGTGAGACAGGACATGAAAGG | 21,22 |
| Claudin-10b | AGACACAGGCTTCTTCCTAGA | GAGTGAGACAGGACATGAAAGG | 21,22 |
| Claudin-11 | CGAACTCCTGGACTCAAAGTATC | GGCTCCCATTGTCATCTGTATC | 23,23 |
| Claudin-14 | CACCAGCTGCCTACAAAGA | GACTCACACGTAGTCGTTCAG | 19,21 |
| Claudin-15 | GAAACCTTTGGCTTCTTCATGG | AGATGGTGTTGGTGGTGATG | 22,20 |
| Claudin-19 | GCAGAAAGATGAGGAGACAGAG | AGAGGGTAGGACCTCTGAATTA | 22,22 |
| Occludin | GGTTCACTTCTCCCAGTCTTTC | AGACACAATCAACAGGGTTAGG | 22,22 |
| Tricellulin | GTACTCGTGGTTGCTGGATTAG | GCCACCAATTAGAGTCCAGAAG | 22,22 |