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. 2018 Nov 29;316(2):L358–L368. doi: 10.1152/ajplung.00345.2018

Fig. 7.

Fig. 7.

Respiratory syncytial virus (RSV) infection induces paracellular permeability and disrupts tight-junction (TJ) structure in cultured mouse tracheal epithelial cell (mTEC) monolayers. Confluent mTEC monolayers were infected with RSV or left untreated. On day 4 post-inoculation, barrier properties of the cells were investigated by either transepithelial electrical resistance (TEER) measurement (A) or fluorescent dextran permeability assay (B). Data are presented as percent change vs. control ± SE; n ≥ 3, *P < 0.05, and ***P < 0.001 vs. control group. C: cells were fixed on day 4 post-infection and immunolabeled for different apical junctional complex (AJC) proteins. Arrows indicate normal TJs and adherens junctions (AJs) in control cell monolayers. Arrowheads point to areas of TJ disassembly in RSV-infected cells. Scale bar, 40 μm. Images are representative of 3 independent experiments.