Fig. 5.

BPI fold-containing group A member 1-deficient (Bpifa1^−/−) polymorphonuclear neutrophils (PMN) do not have impaired chemotaxis response. PMN from wild-type (WT) and Bpifa1^−/− mice were isolated from bone marrrow. Chemotaxis assays were performed using a modified Boyden technique. WT and Bpifa1^−/− PMN were stimulated with PMN chemoattractant N-formyl-methionyl-leucyl-phenylalanine (fMLP; 10⁵ M), keratinocyte chemoattractant (KC; 50 ng/ml), C-X-C motif chemokine ligand 10 (Cxcl10; 100 ng/ml), Cxcl9 (50 ng/ml), and combination of Cxcl10 and Cxcl9. Total PMN migration represented as relative fluorescence units (RFU). ●, WT PMN; ○, Bpifa1^−/− PMN; n = 4–6 wells/group. Generalized linear mixed model: no significant differences observed. Horizontal bars indicate mean values. Data represent 2 experiments.