Fig. 7.

C-X-C motif chemokine ligand 10 (Cxcl10) induction in myeloid cells during inflammation is impaired in BPI fold-containing group A member 1-deficient (Bpifa1^−/−) mice. Top: lung sections from wild-type (WT) and Bpifa1^−/− mice 24 h after intranasal instillation of lipopolysaccharide (LPS). Tissue sections were stained with antibodies against Cxcl10 (red) and F4:80, a marker for macrophages, or Ly6G, a marker that primarily stains polymorphonuclear neutrophils (PMN; both in green). Cell nuclei were stained with DAPI (blue); colocalization of Cxcl10 and cell markers is shown in yellow (white arowheads). Bottom: ratios of Cxcl10+ stained cells to total Ly6G+, F4:80+, or combined Ly6G+ plus F4:80+ cells in tissue sections from mice in these experiments. Each data point represents the average number of cells per field in 10 random fields at ×20 magnification. ●, WT values; ○, Bpifa1^−/− values; n = 3 mice/group. Generalized linear mixed model: *P < 0.05. Data represent 2 experiments.