Fig. 6.

Myeloid differentiation factor 88 (MyD88)-deficient mice have no difference in mucous cell metaplasia in response to ovalbumin (OVA)-mediated T helper cell type 2 inflammation. A: representative images of Ulex europaeus agglutinin I (UEA-1) for mucin (Muc)5ac staining from wild-type (WT) and MyD88 knockout (KO) mouse lungs following OVA-mediated inflammation. Original scale bar, 50 μm. B: Muc5ac expression by mRNA from WT or MyD88-KO mouse lung (n = 3 mice per group). C: immunoblotting for UEA-1 and total protein lysates (B) with corresponding quantification (n = 3 mice per group). Dashed boxes indicate areas of quantification. DAPI, 4′,6-diamidino-2-phenylindole.