Effects on plasma aldosterone of the circadian cycle, standardized behaviors, and their interaction. There were significant endogenous circadian rhythms (n = 16, P < 0.001 using mixed-model cosinor analyses) in plasma aldosterone in all five standardized behaviors (A), significant differences in mean aldosterone level between these five behaviors (B), and a significant interaction between these effects (see different circadian amplitudes, phases, and shapes between behaviors in A).