Fig. 11.

Stimulation of adenosine monophosphate kinase with AICAR increased phosphorylation of aquaporin-2 (AQP2) at serine 256 in rat inner medulla (IM). A: representative Western blot showing AQP2 abundance and phosphorylated AQP2 in rat IM with AICAR stimulation for 15 min. Brackets indicate AQP2 glycosylated protein between 35 and 45 kDa and arrows indicate unglycosylated protein at 29 kDa. Samples from a different rat were loaded into each lane. B: bar graph showing the ratio of the band densities of phosphorylated AQP2 to total AQP2 abundance. Bars  = means ± SE. *P < 0.05 vs. control tissues (290 mosmol/kgH₂O, without AICAR treatment); n = 4 rats/condition.