Fig. 3.

Inflammatory cytokine/chemokine and healing/fibrosis mRNA levels rise at day 2 following cisplatin. Mice were injected with vehicle or cisplatin (30 mg/kg). At 1 or 2 days following cisplatin, mice were sacrificed, and tissues were collected. RT-PCR was performed for mRNA encoding tumor necrosis factor-α (TNF-α, A), interleukin (IL)-1β (B), IL-1α (C), IL-6 (D), C-C motif chemokine ligand 2 (CCL2, E), C-X-C motif chemokine ligand 2 (CXCL2, F), collagen Ia (Col1a, G), transforming growth factor-β (TGF-β, H), and plasminogen activator inhibitor-1 (PAI-1, I) (n = 4–5 for vehicle-treated mice; n = 8 for cisplatin day 1 and day 2 mice). Graphs display means ± SE with each mouse indicated by one open circle. Significance was determined by 1-way ANOVA with Dunnett’s posttest for multiple comparisons with the vehicle-treated group.