Fig. 1.

Na⁺/Ca²⁺ exchanger-1 (NCX1) is overexpressed in smooth muscle-specific NCX1-transgenic (TG) mice. Immunoblots show deendothelialized aortic membrane proteins from control (Ctrl) and heterozygous and homozygous TG mice that overexpress NCX1 specifically in smooth muscle (SM-NCX1-TG mice) (TG/+ and TG/TG, respectively). TG/TG mice were used for all physiological experiments in this study. The NCX1 transgene was randomly inserted into the genome, and the protein was, therefore, greatly overexpressed, as illustrated. Protein samples had to be markedly diluted so that both Ctrl and TG NCX1 bands could be visualized on the same gel (see also Supplemental Fig. S3 in Ref. 23). β-Actin was used as the loading control.