Fig. 11.
Acetylcholine (ACh) cumulative dose-response curves for exposed femoral arteries in control (Ctrl) and transgenic (TG) mice in vivo; ACh was applied by superfusion. A: ACh dose versus artery diameter. Ctrl passive diameter (PD) = 390 ± 19 μm (n = 6); TG PD = 361 ± 16 μm (n = 10). B: phenylephrine-induced vasodilation (as %PD). C: ACh-induced decrease (Δ) in cytosolic Ca2+ concentration ([Ca2+]CYT). Basal [Ca2+]CYT was 230 ± 23 nM in Ctrl mouse arteries and 335 ± 10 nM in TG mouse arteries, but the normalized vasodilation curves (B) and [Ca2+]CYT curves (C) from Ctrl and TG mice were virtually superimposable. Values are means ± SE.