Fig. 5.

Confirmation of GluN2B-knockdown (KD) in mouse spinal cord. GluN2B-floxed mice were injected at time of weaning with saline (control) or adeno-associated virus expressing Cre recombinase (AAV9-Cre; conditional knockdown). Quantitative real-time reverse transcription (RT-qPCR) of spinal cord tissue indicates no changes in Grin2A mRNA levels (A) but a significant decrease in Grin2B mRNA (B) in GluN2B-KD mice compared with controls. C: GluN2B protein was also reduced in spinal cord from GluN2B-KD mice relative to controls. *P < 0.05; **P < 0.01, significant difference from saline control (unpaired Student’s t-test).