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Femoral nitrotyrosine expression normalized to β-actin (A), total aortic NOX4 protein expression normalized to β-actin (B), aortic Gp91phox normalized to β-actin (C), and representative Western blots (D). Values are means ± SE; n = 8 or 9 per group. Data analysis was performed with a 1-way ANOVA with a Tukey’s post hoc test. HS, high salt; NS, normal salt; VWR, voluntary wheel running. α vs. NS, †vs. NS-VWR, ‡ vs. HS-VWR (P < 0.05).
