Sections were stained as indicated with e-cadherin (green: A-D) and SUMO2/3 (red: A-H) in animals subjected to sham procedure (A,E), or 15 (B,F), 30 (C,G), and 45 minutes (D,H) of ischemia. Additional sections were stained for e-cadherin (green: I, K) and SUMO1 (red: I-M) after sham procedure (I, J) or 45 minutes of ischemia (K, M). Reperfusion period was 30 minutes in all I/R animals. Magnification 200x, size bar denotes 50μm.