Mucosal scrapings from small intestines of WT or Ubc9Tg animals were analyzed by Western blot for SUMO1 (A), SUMO2/3 (B), Ubc9 (C), and bActin (D) protein levels. Markers denote molecular weight in kilo Dalton. Small intestinal sections from wild type (E,G) or Ubc9Tg animals (H,F) were stained for Ubc9 (red: E,F) or SUMO2/3 (red: G,H) as well as e-cadherin (green: E-H). Magnification 200x. Inserts detail the villus tip staining for Ubc9 or SUMO2/3 respectively.