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. Author manuscript; available in PMC: 2019 Mar 2.
Published in final edited form as: Lab Invest. 2018 Feb 22;98(6):799–813. doi: 10.1038/s41374-018-0035-6

Figure 5: Modulation of villus-tip epithelial responses in Ubc9Tg animals after I/R.

Figure 5:

Intestinal epithelial villus-tip epithelia from ischemic regions of wild type and Ubc9Tg (45 minutes of ischemia and 3 hours of reperfusion) were isolated by laser microdissection and capture and RNA was isolated to perform a cytokine targeted PCR array. A) Scatter and B) Volcano- blot outline the distribution of regulated genes, significantly up- and downregulated genes are listed in C). D) IL17A protein levels in non-ischemic small intestinal loops (NI) and loops subjected to ischemia/reperfusion (IR; 45minutes ischemia and 6 hours or reperfusion). Quantification was performed by densitometry and is represented as the ratio of IL17A to bActin band intensity. n=5; *p≤0.05