Table 2.
Time of incubation, dilution and manufacturer of secondary antibody and developing time used in the P22 ELISA
| Secondary antibody | OPD | ||||
|---|---|---|---|---|---|
| Species | Antiserum | Dilution | Time (min) | Manufacturer | Time (min) |
| Cattle | Rabbit anti bovine IGg(H/L)-HRP | 1/4000 | 30 | Biorad(Hercules, USA) | 6 |
| Goat | Rabbit anti sheep IGg(H/L)-HRP | 1/2000 | 30 | SoutherBiotech(Birmingham, USA) | 15 |
| Sheep | Rabbit anti sheep IGg(H/L)-HRP | 1/2000 | 30 | SoutherBiotech(Birmingham, USA) | 6 |
| Pig | Goat anti-pig IgG-Fc Fragment-HRP | 1/20000 | 45 | Bethyl Laboratories(Montgomery, USA) | 15 |