Fig. 4.

Changing the metabolic state predictably alters sensitivity to the deacetylase inhibitor—vorinostat. a Schematic of the phenotype microarray analysis. HeLa cells were cultured in different metabolic conditions and exposed to vorinostat or control (DMSO). The metabolic state and acetylation flux in each condition were determined using FBA. b The acetylation flux (mmol/gDW cells/h) correlated significantly with the extent of growth inhibition by vorinostat. The scatter plot shows the predicted acetylation flux in each metabolic condition and the ratio of the area under the growth curve (AUC) for vorinostat treatment relative to control. Conditions with lower ratio (< 1) were more sensitive to vorinostat and showed higher acetylation flux. Average of four replicates shown