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. 2019 Mar 1;38:109. doi: 10.1186/s13046-019-1112-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Estrogen reverses TGF-β-induced EMT in a miR-1271 dependent manner. a The expression of miR-1271 in T47D cells after treatment with TGF-β1 at indicated concentration. b Transwell invasion assay of miR-1271-depleted T47D or control cells with TGF-β1 or/and E2 treatment. c Luciferase reporter analysis of TGF-β signaling activity in cells as in (B). d Localization of SMAD2 in cells as in (B) as determined by immunofluorescence staining. e The expression of pSMAD2 in cells as in (A) by western blotting. f The expression of SNAI1/2, TWIST1 and ZEB1 in cells as in (A) as determined by RT-qPCR. *P < 0.05. Scale bar, 50 μM