Fig. 6.

ERα transactivates the miR-1271 expression. a Promoter analysis of miR-1271. Four half EREs were located on the core promoter region of miR-1271 (− 1100 to + 1). b The miR-1271 promoter activity was measured in T47D cells with E2 treatment as determined by luciferase analysis. c Interaction between ERα and the miR-1271 promoter sequence in T47D cells with or without E2 treatment as determined by ChIP assay. d The miR-1271 promoter activity was measured in ERα-transfected MDA-MB-231 cells with or without E2 treatment as determined by luciferase analysis. e The expression of miR-1271 in T47D cells with E2 treatment (left), ERα-depleted T47D cells, as well as in control cells as determined by RT-qPCR. f The expression of miR-1271 in ERα-transfected MDA-MB-231 cells with or without E2 treatment as determined by RT-qPCR. *P < 0.05