Fig. 3.

Elimination of all three cortical formins is detrimental for cell division and development. (A) Inactivation of the forA, forE, and forH genes in the triple-KO mutant was verified by immunoblotting using specific formin sera. Porin was used as a loading control. (B) WT and the mutant cells indicated were grown for 48 h in shaken suspension at 150 rpm, subsequently seeded on glass coverslips, fixed, and stained with DAPI to visualize the nuclei. (Scale bar, 20 µm.) (C) Quantification of nuclei in cells as shown in B. n, number of analyzed cells. (D) Coincident elimination of ForA, ForE, and ForH blocks development. WT or formin-deficient cells were transferred with a tooth pick onto a lawn of Klebsiella aerogenes on nonnutrient agar plates and monitored after 96–120 h of development. (Scale bar, 0.5 mm.)