Fig. 3.

Daptomycin–membrane interactions. The neutron reflectivity profiles (symbols) and fits (lines) of (A) A8819 and (B) A8819_Cls2T33N membrane models in D₂O and H₂O are shown. The NR profiles in H₂O are offset for clarity. The arrows indicate fringe shifts of NR profiles. The molecular models of reconstituted (C) A8819 and (D) A8819_Cls2T33N membranes show a thicker but more CL-rich membrane structure (with CL in yellow, PG in red, and L-PG in blue). Small-angle neutron scattering profiles measured for (E) A8819 and (F) A8819_Cls2T33N membranes treated with and without daptomycin (4 μg/mL). The arrow indicates the lipid bilayer, whereas the star indicates the Bragg peak as the sign of micelle formation.