Fig. 1.
Progerin expression reduces the diffusional mobilities of a subset of nuclear envelope proteins. (A) Normalized FRAP in the nuclear envelope for indicated EGFP-tagged proteins in control (WT, green) and HGPS fibroblasts (red). The t1/2 of FRAP are used for statistical tests. (B) Normalized t1/2 of FRAP of EGFP-tagged mN2G, SUN2, and emerin in HGPS fibroblasts treated with 2.5 µM FTI 277. Values are normalized to cells treated with dimethyl sulfoxide (DMSO). (C) Normalized t1/2 of FRAP of EGFP-tagged mN2G and SUN2 in NIH 3T3 fibroblasts expressing RFP-tagged lamin A or progerin. See SI Appendix, Table S2, for absolute numbers for t1/2. Data are mean ± SEM from ≥3 experiments (n > 15 cells). n.s., P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001 by Student’s t test.