Fig. 3.

Manipulation of EZH2 expression affected EC angiogenesis in vitro. (A) Overexpression of EZH2 in normal ECs led to a decreased number of nodes and tubes in Matrigel tube formation assay. Knockdown of EZH2 in normal ECs (B) and diffuse cutaneous SSc ECs (C) resulted in increased angiogenesis. (D) Inhibition of EZH2 by DZNep similarly increased angiogenesis in diffuse cutaneous SSc ECs compared with cells treated with PBS control. Results are expressed as mean ± SD. Mann–Whitney U test was performed, and *P < 0.05 was considered significant.