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. 2019 Feb 7;116(9):3712–3721. doi: 10.1073/pnas.1820414116

Fig. 2.

Fig. 2.

Differential localization of wild-type (G0) APOL1 to LDs and its risk variants (G1, G2) primarily to the ER. (A) SR-SIM micrographs of representative primary human podocytes transiently transfected with APOL1-RFP, labeled for perilipin-2 (light blue), and with BODIPY 493/503 and DAPI (blue). [Scale bars, 10 µm and 2 µm (Insets).] (B) SR-SIM micrographs of primary human podocytes transiently transfected with APOL1-GFP, labeled for mitochondria (MitoTracker in red), ER (calnexin in magenta), and DAPI (blue). [Scale bars, 10 µm and 2 µm (Insets).] (C) Colocalization analysis of APOL1-GFP with MitoTracker Red (mitochondria) or calnexin (endoplasmic reticulum). Data are presented as means ± SD. *P < 0.05. (D and E) Yellow arrow indicating colocalization of APOL1-RFP with GFP-Rab7. Airyscan micrographs of live primary human podocytes coexpressing APOL1-RFP (G0) with GFP-Rab7 and mito-BFP (D) or with GFP-PLIN2 and BFP-KDEL (E). [Scale bars, 10 µm and 2 µm (Insets).] See SI Appendix, Fig. S3 for time frame still images. See also Movies S1 and S2.